Chromatography Online
November 12th 2024. Liquid chromatography and tandem mass spectrometry (LC–MS/MS) was used to show that repeated stress can cause delayed but sustained changes in blood plasma …
Back to Basics: The Role of pH in Retention and Selectivity
Jan 1, 2017 · Peak 3 (orange) has a similar p Ka (2.75), so very similar behavior is seen for peaks 3 and 4, with stable retention above pH 4.0. Although the retention times of these two peaks …
LCGC North America - Chromatography Online
LCGC North America. ... Practical advice, tutorials, and peer-reviewed research on liquid chromatography (HPLC), gas chromatography (GC), mass spectrometry (MS) and related …
Good Laboratory Practice (GLP): An Overview for the ...
Oct 1, 2023 · Good Laboratory Practice (GLP) is a regulatory requirement to help ensure the quality, reliability, and integrity of pharmacological and toxicological studies during drug …
Troubleshooting Basics, Part IV: Peak Shape Problems
Jul 1, 2012 · A buffer concentration of 5–10 mM usually is adequate to buffer the mobile phase, column, and injection solvent in reversed-phase separations. If buffer concentration problems …
Chromatography Fundamentals, Part V: Theoretical Plates
Nov 1, 2018 · Conclusions. The number of theoretical plates, which forms the basis of chromatographic theory, is a key parameter used in all modes of chromatography for …
Reversed-Phase HPLC Mobile-Phase Chemistry — Explained
Nov 1, 2014 · Table I: Solvochromatic and physicochemical properties of common solvents used in reversed-phase HPLC. Modern approaches to working with ionizable analytes often involve …
Understanding Gradient HPLC - Chromatography Online
Jul 1, 2013 · Gradients in reversed-phase HPLC usually involve the on-line (dynamic) mixing of solvents to achieve a steady increase in the organic solvent (typically methanol or acetonitrile) …
Peak Shapes and Their Measurements: The Need and ...
Dec 1, 2017 · Volume 35. Issue 12. Pages: 846–853. A number of approaches for peak shape measurement are available in modern chromatography software. We discuss the advantages …
Chromatography Fundamentals, Part VIII: The Meaning ...
Nov 1, 2019 · The general resolution equation is defined as. where â s is the spacing between the apex of two signals with respect to their average baseline width, wav = (w1 + w2)/2. This …